From single reads to complete double strand sequences – Eurofins MWG Operon has the answer for all kind of DNA sequencing requirements.
With over 10 years experience in Sanger sequencing by using the latest state-of-the-art ABI 3730xl 96-capillary DNA Analyzers you can rely on high quality results in best possible time.
The Value Read Tube service is the service of choice for fast and reliable standard DNA sequencing reactions in tube format. It is highly automated to allow rapid processing of plasmids and PCR products. Sequencing results are delivered within 20 hours from receipt of your samples.
Samples can be sent as clones, un-purified PCR products and purified DNA.
Convenient online functionalities and Prepaid Barcode Labels complete the Value Read Tube service.
For single read sequencing of plasmids and PCR products in 96well microtiter plates Eurofins MWG Operon offers the Value Read Plate DNA sequencing service. Samples can be sent as clones, un-purified PCR products and purified DNA. Plasmid DNA preparation and PCR clean-up is included in the plate sequencing service.
Convenient online ordering and Prepaid Plate Coupons complete the Value Read Plate service.
This service is offered for pre-cycled sequencing reactions in single tubes or in plates. Eurofins MWG Operon carries out the dye terminator removal and run the DNA sequences on ABI 3730xl DNA analyzers under standard parameters.
As the name implies, this DNA sequencing service is totally customised to your requirements. Beside individual support, the Service à la Carte includes the option of selectable setups “á la carte” for sample preparation, sequencing reactions and data analysis.
It is available for all kind of template types and is particularly recommended for
- Large constructs, phage or genomic DNA,
- Sequences with GT repeats or hairpin structures,
- Templates like shRNA, 16S rRNA or GC rich DNA sequences,
- DNA samples with unknown concentration and
- Sequencing reactions with primers where the
Tm < 50°C or Tm > 65°C.
For all sequence analyses of regions which are larger than the read length of a single read we have:
All our Sequencing services include
- Convenient online ordering via Barcode Labels
- Individual order settings within your "Personal Preferences"
- Free Primer Design Tool
- Free standard vector primers
- Read lengths of up to 1100 Q20* bases
- Validation by internal quality control standards (DIN EN ISO 9001:2000)
- Optional BLASTX and/or Heterozygote analysis included
- Secure download of trace files in *.scf or *.ab1 format by using “Chromas”
- Clipped and unclipped sequence results in FASTA format
- Unique “Group Administration Tool”
- Quality report as *.pdf file for free
- Storage of sequence data for 100 days
- Free storage of custom synthesised primers
- Order tracking and history with convenient re-order option
- Prepayment services with discount opportunities
1 Q20: quality bases with a minimal medium score of 20 calculated by ABI’s Kb-base caller (comparable with PHRED)
