Custom DNA Sequencing - From single reads to complete double strand sequences – Eurofins MWG Operon has the answer for all kind of DNA sequencing requirements.
With over 10 years experience in Sanger sequencing by using the latest state-of-the-art ABI 3730xl 96-capillary DNA Analyzers you can rely on high quality results in best possible time.
Value Read Tube service
The Value Read Tube service is the service of choice for fast and reliable standard DNA sequencing reactions in tube format.
- It is highly automated to allow rapid processing
- Sequencing results are delivered next day after samples received
- One repetition in case of technical failure included
Your samples can be sent as plasmid DNA, plasmid clones, un-purified and purifid PCR products and premixed samples
Convenient online functionalities and Prepaid Barcode Labels complete the Value Read Tube service.
Service à la Carte
As the name implies, this DNA sequencing service is totally customised to your requirements. Beside individual support, the Service à la Carte includes the option of selectable setups “á la carte” for sample preparation, sequencing reactions and data analysis.
It is available for all kind of template types and is particularly recommended for
- Large constructs, phage or genomic DNA,
- Sequences with GT repeats or hairpin structures,
- Templates like shRNA, 16S rRNA or GC rich DNA sequences,
- DNA samples with unknown concentration and
- Sequencing reactions with primers where theTm < 50°C or Tm > 65°C.
Value Read Plate
For single read sequencing of plasmids and PCR products in 96well microtiter plates Eurofins MWG Operon offers the Value Read Plate DNA sequencing service.
Samples can be sent as plasmid DNA, plasmid clones, un-purified and purfied PCR products Plasmid DNA preparation and PCR clean-up is available as additional plate sequencing service.
Convenient online ordering and prepayment services complete the Value Read Plate service:
Prepaid Plate Kits
Each Prepaid Plate Kit comes with a 96well plate and the appropriate seals in a metallic sample box. For each template type we offer the perfect Prepaid Plate Kit:
- Plasmid DNA, purified PCR products or premixed samples
- Unpurified PCR products incl. PCR clean-up and
- Plasmid clones as stab culture incl. DNA preparation
Prepaid Plate Coupons
Prepaid Plate Coupons are code numbers provided electronically in your online account. You can use them:
- For a second prepaid sequencing run in addition to one of our Prepaid Plate Kits (e.g. forward & reverse sequencing reads).
- As alternative to the Prepaid Plate Kits for plasmid DNA, purified PCR products or premixed samples for immediate use.
For all sequence analyses of regions which are larger than the read length of a single read we have:
This service is offered for pre-cycled sequencing reactions in single tubes or in plates. Eurofins MWG Operon carries out the dye terminator removal and run the DNA sequences on ABI 3730xl DNA analyzers under standard parameters.
All our Sequencing services include
- Convenient online ordering via Barcode Labels
- Individual order settings within your "Personal Preferences > "
- Free Primer Design Tool
- Free standard vector primers
- Read lengths of up to 1100 Q20* bases
- Validation by internal quality control standards (DIN EN ISO 9001:2000)
- Optional BLASTX and/or Heterozygote analysis included
- Secure download of trace files in *.scf or *.ab1 format by using “Chromas”
- Clipped and unclipped sequence results in FASTA format
- Unique “Group Administration Tool >”
- Quality report as *.pdf file for free
- Storage of sequence data for 100 days
- Free storage of custom synthesised primers
- Order tracking and history with convenient re-order option
- Prepayment services with discount opportunities
1 Q20: quality bases with a minimal medium score of 20 calculated by ABI’s Kb-base caller (comparable with PHRED)
