- It is possible to send either a stab or glycerol culture with the selected E.coli clone, the purified plasmid DNA or your unpurified or purified PCR product
- Please use 1,5 ml tubes for your samples
- If available, please send a reference sequence in electronic form
Minimum concentration:
Plasmid DNA: 100 ng/µl
PCR products: 10 ng/µl
Minimum total amount:
- Single Strand Sequencing:
Plasmid DNA: 1 µg/1 kb insert
PCR products: 100 ng/1 kb PCR product
- Double Strand Sequencing:
Plasmid DNA: 2 µg/1 kb insert
PCR products: 200 ng/1 kb PCR product
- 4fold Coverage Sequencing:
Plasmid DNA: 4 µg/1 kb insert
PCR products: 600 ng/1 kb PCR product