Gene Evolution Libraries are ideally suited for screening approaches to improve the properties of your protein of interest. Eurofins MWG Operon offers production of mutations in single positions, multiple positions within specific domains or random substitution using degenerated oligonucleotides (wobble oligonucleotides).
With this technology, nucleotides can be specifically predetermined and modified. Finally the desired mutants are represented in the library. Our proprietary software GENEius provides unique features for gene optimisation. Libraries will be delivered linear or cloned.
1. Consultancy by our Experts (optional)
To find the optimal expression system, to check your sequences in advance and to design your perfect genes
2. Sequence Optimisation (optional)
By using our proprietary software GENEius. The supplied amino acid or DNA sequences will be optimised, e.g. by adapting to the codon usage of any organism. Repeats or hairpin structures are avoided and the GC content is adjusted. GENEius is also available as free online tool for our customers
3. Experimental Design
Of overlapping oligonucleotides for gene synthesis by using GENEius
4. Oligonucleotide Synthesis
At our in-house DNA synthesis facility
5. Gene Synthesis
Of new genes from only a few base pairs up to several kb in size. Synthetic genes are assembled by using overlapping oligonucleotides, employing LCR (Ligase Chain Reaction), and PCR technologies
6. Generation of Gene Mutant Libraries
Either by gene synthesis or by site directed mutagenesis
7. Subcloning
Into a standard vector or into a vector of choice
8. Quality Control
By double strand DNA sequencing of a certain number of clones.
Your Gene Evolution Library will be delivered as cloned library.You receive a quality assurance report, including detailed project specifications, complete plasmid description, restriction map and DNA sequence information.
We ensure strict confidentiality of every project. The high quality standard of our service is permanently assured by a professional quality management. We are certified according to DIN EN ISO 9001:2000.
