Eurofins MWG Operon offers standard genes which are available in any size, with moderate GC content (40-65%), without extensive repeats (> 20 bp) or critical hairpin structures and without extensive homopolymer stretches (> 20 bp). Our proprietary software GENEius provides unique features for gene optimisation.

1. Sequence Optimisation (optional)
By using our proprietary software GENEius. The supplied amino acid or DNA sequences will be optimised, e.g. by adapting to the codon usage of any organism. Repeats or hairpin structures are avoided and the GC content is adjusted. GENEius is also available as free online tool for our customers.

2. Experimental Design
Of overlapping oligonucleotides for gene synthesis by using GENEius

3. Oligonucleotide Synthesis
At our in-house DNA synthesis facility

4. Gene Synthesis
Of new DNA sequences from only a few base pairs up to several kb in size. Synthetic DNA is assembled using overlapping oligonucleotides, employing LCR (Ligase Chain Reaction) and PCR technologies

5. Subcloning
Into a standard vector or on request into a vector of choice

6. Quality Control
By double strand DNA sequencing of the cloned gene performed at our in-house sequencing facility.

Genes will be delivered by using E. coli clones and plasmid DNA (mini scale preparation). Higher scales are available on request. You receive a quality assurance report, including detailed project specifications, complete plasmid description, restriction map and DNA sequence information.

We ensure strict confidentiality of every project. The high quality standard of our service is permanently assured by a professional quality management. Eurofins MWG Operon is certified according to DIN EN ISO 9001:2000.