Re-Sequencing is the sequencing of pre-sequenced and pre-selected genomic regions such as exons in order to find a correlation between sequence deviations, for instance SNPs or deletions and phenotypes.
Eurofins MWG Operon offers a full service in 3 phases from (I) primer design, primer synthesis and establishment of PCR conditions, (II) to high-throughput PCR and double strand sequencing concluding with bioinformatic analysis, (III) to determine differences between reference and samples.
Phase I: Establishment of the PCR
- Primer design for all exon regions
- Primer synthesis in HPSF® quality
- Establishment of PCR amplification on two DNA samples
- Double strand sequencing of the PCR fragments
- Two repetitions of PCR setup in the event of failure
Phase II: PCR and Sequencing
- High throughput PCR of all samples
- Purification of PCR products
- High throughput sequencing of PCR products
- One repetition in the event of failure
Phase III: Bioinformatic Analysis
- Double Strand sequencing recommended
- Identification of all differences between reference and samples
- Homozygous substitutions
- Heterozygous substitutions
- Detection of insertions/deletions
- Mutation report and comparison of all samples provided.
- Reliable analysis of genetic variations
- High quality results and concise analysis format
- Fast turnaround times
- Consultancy and project support
- Guaranteed project confidentiality.
