Sequencing of cDNA or SAGE clones by Sanger technology with read lengths up to 1100 base pairs.

Ultra-deep sequencing of cDNA-, SAGE-, miRNA- and sncRNA-libraries with the Genome Sequencer™ FLX.

Sequencing of EST or SAGE Clones by Sanger Technology

• Clones can be delivered as stab or glycerol cultures in plates
• Delivery of cDNA libraries as ligation- or transformation mix possible
• High throughput DNA preparation and purification
• High throughput sequencing with Sanger technology
• Read length up to 1100 bases
• Sequencing from the 5’-, the 3’- or both ends
• Professional clustering and assembly
• Additional bioinformatic services on request
• High quality sequence data
• Clones for further experiments available.

Sequencing of cDNA Libraries by GS FLX

• Ultra Deep Sequencing of libraries
• No cloning required, therefore no cloning bias
• Sequencing from the 5’-, the 3’-end or shotgun sequencing is possible
• No arrays necessary for expression studies
• Detection of transcripts that are not on commercially available arrays.

• Ligation and transformation service included
• Experience of over 100 large EST projects
• cDNA library service available: “From tissue to the transcriptome”
• No array necessary for expression studies.