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FAQ Genome Sequencing

Ultra Deep Sequencing of Exons and|Genome Fragments

Sequencing of PCR amplicons with the Genome Sequencer FLX
Ultra deep sequencing is a useful new method and can be used for several applications, including the detection of rare mutations or the study of methylation patterns.

We can assist you with the design of the amplicons or we can do the design work as part of our service offering. Eurofins MWG Operon offers amplicon sequencing by Genome Sequencer™ FLX technology.

Specifications
  • Delivery of amplicons with required adaptor sequences
  • Pooling of samples is possible with specific sequence tags (barcodes)
  • Generation of a GS shotgun library (optional) and emPCR
  • Ultra deep sequencing with the GS FLX
  • Sorting of the sequences (assembly, if required).
Your Advantages
  • Support in experimental design
  • Detection of rare somatic mutations
  • Detection of SNPs in candidate populations
  • Methylation studies.