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Fragment Length Analysis For Genotyping STRs, |VNTRs, Microsatellites or DNA Marker

Rely on proven expertise for DNA fingerprinting and fragment length analysis for research, food authenticity and DNA forensics.

Highest accuracy is ensured
All instruments used for fragment length analysis fulfil the highest demands of a forensic DNA lab. Our laboratory equipment is thoroughly monitored and the working routines are fully documented.

Expertise and success also with challenging samples
Acknowledged scientists and a highly educated technical staff are responsible for DNA profiling and microsatellite analyses. Their expertise is requested by courts, police departments and many food and biotech companies.

Trusted reliability due to long term expertise
10 years of experience and 180,000 DNA samples per year illustrate our strength in genotyping DNA markers and profiling STRs.

Flexible capacities lead to fast results
An extensive range of equipment with back up machines and sophisticated software solutions ensure fastest turnaround times for every genotyping request.

Fragment length analysis - Ready to Load

Your fragments are fully prepared to perform the CE

  • Separation of fragments (100 bp - 550 bp) by capillary electrophoresis (CE) on ABI 3130 XL sequencing machine under standard conditions
  • Results as FSA files by Email (GeneMapper analysis software required)
  • Larger fragments on request

Fragment length analysis service

You just do the PCR, we do the rest

  • Optimal dilution of the PCR products and addition of loading dye and size standard
  • Separation of the fragments by capillary electrophoresis on ABI 3130 XL sequencing machine
  • Data analysis
  • Results as XLS and FSA files by Email

Full service genotyping project

We carry out your complete project

Plan your genotyping project with us and take advantage of our experience in DNA extraction, marker selection and assay design. We can assist in every stage of your project:

  • Literature and database search
  • Expert advice on typing strategy and primer design
  • Validation of variable regions
  • Marker selection, assay design and primer synthesis
  • DNA extraction and amplification
  • Singleplex or multiplex PCR
  • Evaluation and documentation of results

Matrix, Filter Sets and Dye Combinations:
FAM, NED, HEX and ROX or FAM, JOE, TAMRA and ROX (ABI Standard Dye Set DS-30)  
FAM, VIC, NED and ROX (ABI Standard Dye Set DS-31) 
FAM, JOE, NED and ROX (ABI Standard Dye Set DS-32) 
FAM, VIC, NED, PET and LIZ (ABI Standard Dye Set DS-33) 
FAM, Yakima Yellow, ATTO 550, ATTO 565 and ATTO 663 (Eurofins alternative dye set EF-01) 


 

Important Documents

Application note >
Multiplex microsatellite analysis with 5’ dye-labelled marker sets on an ABI analyzer.

Sample submission guide >
How to send your samples for fragment length analysis

Service flyer >
Overview of our fragment length analysis service as PDF

Primer Labelling

Fluorescent dyes >
Select the best fluorescent label for specific assays

ATTO dyes >
Alternative dyes from Atto-Tec for labelling your primers

Quality Certificates

Our quality management is ISO 9001:2008 certified. The laboratory is accredited according to ISO 17025:2005 for forensic genetics and food authenticity.