Site directed mutagenesis’ comprise the incorporation of one base substitutions, mutagenesis of multiple bases, deletions or insertions and random mutagenesis for applications as

• Generation of Protein Variants
  for studying protein structure function relationships or for optimising enzymes
• Optimisation of functional Efficiency
  of regulatory elements (promoter, enhancer, splice sites)
• Correction of PCR based Errors
  e.g. errors within cloned PCR products
• Randomisation of Codons
  Parallel generation of all 20 amino acid variants at one site of interest
• Random Mutagenesis
  Error-prone PCR to create random mutant libraries
• Multiple Mutagenesis
  Multiple introduction of different mutations in one DNA fragment.

• Consulting
  Competent assistance in modifying gene sequences (e.g. optimisation of codon usage)
• Experimental Design
  Selection of optimal methods specific for each project and design of mutator oligonucleotides needed for in vitro mutagenesis
• In vitro Mutagenesis
  Implementation of optimised methods for introducing single or multiple mutations (base substitutions, deletions, insertions) into cloned gene fragments
• Gene Verification
  Each mutated gene sequence is verified by double stranded DNA sequencing to ensure 100% accuracy of in vitro mutagenesis
• Delivery and Documentation
  The mutagenised fragments will be delivered as E. coli clones and plasmid DNA (mini scale preparation), higher scales are available on request (link plasmid preparation). We provide a quality assurance report, including all experimental data of the plasmid, e.g. all sequencing information.

We ensure strict confidentiality for every project. The high quality standard of our service is permanently assured by a professional quality management. We are certified according to DIN EN ISO 9001:2000.

Feel free to contact us by email  or by our online contactform for further information or individual discussion of your project – we’ll help you find the best solution. Our experts will get in touch with you immediately.