Our service covers all steps in quantitative PCR (qPCR) and SNP detection - from the development of the experimental strategy to the final data analysis. For the performance we use according to the project, validated probes, customised probe designs or SYBR green assays.

• Gene Expression Analysis
  Using absolute and relative mRNA quantification. For absolute quantification, Eurofins MWG Operon uses internal standards, e.g. synthetic produced fragments. For relative quantification different controls can be used, e.g. expression of housekeeping genes 
• Analysis of Oncogene Amplification
  Quantification of gene copy numbers of oncogenes in relation to the whole genome
• SNP Genotyping / Polymorphism Analyses
  Differentiation of polymorphism via specific probes, e.g. for the study of genetic diseases.

• Consulting
  Comprehensive support in the selection of project oriented strategies
• Experimental Design
  Design of gene specific DNA primers and qPCR probes. Design, cloning and synthesis of gene specific DNA and RNA standards
• Sample Preparation
  Isolation and purification of DNA and RNA from blood, tissue, cells, food, biotechnology products or buccal swabs
• Assay Development
  Optimisation of new Real-Time PCR assays reaching maximum levels of sensitivity, accuracy and reproducibility
• Routine Assay Performance
  Professional performance of optimised Real-Time PCR assays. Eurofins MWG Operon is able to perform assays with high throughput capacity. Micro fluidity formats are implemented in our processes
• Data Analysis
  We offer complete analysis of raw data for relative or absolute mRNA and/or gene quantifications. Clear results are determined by calculating the ratio of the gene of interest to the housekeeping gene
• Documentation
  The experimental strategy, raw data and final results are summarised in a detailed report.

We ensure strict confidentiality for every project. The high quality standard of our service is permanently assured by a professional quality management. We are certified according to DIN EN ISO 9001:2000.

Feel free to contact us by email  or by our online contactform for further information or individual discussion of your project – we’ll help you find the best solution. Our experts will get in touch with you immediately.