Next generation sequencing services: fast turnaround times using latest technology standards – such as the PacBio RS system, the Illumina HiSeq 2000 system and the Roche GS FLX+ system, operating now with increased chemistry version and Sanger-like length.

By including the Single Molecule Real Time (SMRT) DNA sequencing technology from Pacific Biosciences in our sequencing service portfolio, you have now access to the latest NGS technology on the market.

Current average read length is more than 1000 bp with more than 10% of reads in a range of 1500 bp to 2500 bp and some reads already longer than 4500 bp. With the recent technical updates the sequencer delivers approx. 35 Mbp sequencing data per SMRT cell.

Because of the long read length we will use the SMRT technology initially for de novo sequencing of viruses, bacteria and fungi as well as for resequencing and scaffolding projects.

Illumina HiSeq 2000 delivers up to 576 Gb in 2 flow cells per sequencing run with the 2x100 bp module. Therefore, HiSeq 2000 is the ideal solution for resequencing of genomes or exomes where reference sequences are available.

We offer a broad HiSeq 2000 portfolio which guarantees cost efficient sequencing data for your applications:

• Genome sequencing >
  (Shotgun and Mate-Pair libraries)
  
• Targeted resequencing of sequence capture experiments >
• Illumina mRNA sequencing
• Illumina ChIP sequencing
• Illumina small RNA sequencing

Roche GS FLX+ sequencing is the technology of choice for de novo sequencing of genomes of any size. It is now available with Sanger-like read length with up to 750 bp per read.
The long read length together with our well established expertise in long paired end (LPE) libraries from 3 kbp to 40 kbp, guarantees best possible quality of assembly data. With the addition of the GS Junior to our sequencer fleet, we also offer small scale projects with even faster turnaround times.

Typical projects of our customers are:

• Genome sequencing >
  (Shotgun and long paired end libraries)
• Transcriptome sequencing >
  (Normalised and 3’-fragment libraries)
  
• Small RNA sequencing
• Amplicon sequencing >

Depending on your final objective, we will assist you in designing the appropriate sequencing experiment for your research. In close consultation with you, we will work out the strategy to deliver the best possible data quality.

Are you interested in an individual quote? Need more information? Just contact us! >