High throughput end sequencing of clones from both sides by state-of-the-art Sanger technology on ABI 3730XL sequencers, e.g. the creation of physical maps (minimal tiling path) or scaffolding of contigs and gap closing.

Coverage sequencing of defined BAC clones by Genome Sequencer™ FLX or ABI 3730 XL technology and closing of gaps by primer walking.

High Throughput Screening by End-Sequencing of BAC, PAC, Fosmid or Cosmid Clones by Sanger Sequencing

• Clones can be delivered as stab or glycerol cultures in microtiter plates
• Preparation of the clones and sequencing from both ends
• Ideal for creating physical maps (minimal tiling path)
• Ideal for scaffolding of contigs in genome sequencing projects.

Coverage Sequencing of BAC Clones by GS FLX

• Clones can be delivered as stab or glycerol cultures
• DNA preparation
• Generation of the shotgun library and emPCR (emulsion PCR)
• High throughput sequencing
• Assembly of the sequence reads.

Physical Gap Closure of BAC Clones

• Scaffolding and final gap closure by various PCR approaches and primer walking steps on the BAC DNA
• Additional bioinformatic services on request.

Shotgun Sequencing of BAC, PAC, Cosmid, Fosmid Clones by Sanger Sequencing

• Clones can be delivered as stab or glycerol cultures
• DNA preparation
• Generation of shotgun plasmid libraries
• High throughput Sanger sequencing until requested coverage is reached
• Assembly of the sequence reads.

Physical Gap Closure

• Final gap closure by various PCR approaches and primer walking steps on the BAC, PAC, Cosmid or Fosmid DNA
• Additional bioinformatic services on request.

• Fast turnaround times
• Experience resulting from the completion of more than 1500 BAC sequencing projects
• Project consultancy and support
• Storage of material and data
• Bioinformatic services.