High throughput end sequencing of clones from both sides by state-of-the-art Sanger technology on ABI 3730XL sequencers.

Eurofins MWG Operon is offering coverage sequencing of defined BAC clones by Genome Sequencer™ FLX Titanium reagents or ABI 3730 XL technology and closing of gaps by primer walking.

We offer High Throughput Screening by End-Sequencing of BAC, PAC, Fosmid or Cosmid Clones by Sanger Sequencing

• Clones can be delivered as stab or glycerol cultures in microtiter plates
• Preparation of the clones and sequencing from both ends
• Ideal for creating physical maps (minimal tiling path)
• Ideal for scaffolding of contigs in genome sequencing projects.

Coverage Sequencing of BAC Clones by GS FLX

• Clones can be delivered as stab or glycerol cultures
• DNA preparation
• Generation of the shotgun library and emPCR (emulsion PCR)
• High throughput sequencing
• Assembly of the sequence reads.

Physical Gap Closure of BAC Clones

• Scaffolding and final gap closure by various PCR approaches and primer walking steps on the BAC DNA
• Additional bioinformatic services on request.

Shotgun Sequencing of BAC, PAC, Cosmid, Fosmid Clones by Sanger Sequencing

• Clones can be delivered as stab or glycerol cultures
• DNA preparation
• Generation of shotgun plasmid libraries
• High throughput Sanger sequencing until requested coverage is reached
• Assembly of the sequence reads.

Physical Gap Closure

• Final gap closure by various PCR approaches and primer walking steps on the BAC, PAC, Cosmid or Fosmid DNA
• Additional bioinformatic services on request.

• Fast turnaround times
• Experience resulting from the completion of more than 1500 BAC sequencing projects
• Project consultancy and support
• Storage of material and data
• Bioinformatic services.