Get an excellent insight in your regulome of interest with our services in small RNA sequencing and ChIP sequencing.

Regulation of gene expression in eukaryotic cells takes place on different levels, for example:

• Histone modification
• Transcription factor binding
• mRNA degradation mediated by small RNAs

Small RNAs constitute a family of RNA species that have been demonstrated to play a central role in regulating gene expression in many organisms. With the enormous depth reached by next generation sequencing technologies, you can get a comprehensive view of the small RNAs in your samples.

For this purpose, we prepare a short insert cDNA library for each sample and subsequently sequence these libraries using Illumina HiSeq 2000 technology. To sequence several small RNA samples in one channel in parallel, we offer library preparation with individual barcodes. Routinely we pool up to 12 samples.

Typical projects are:

• Characterization of small RNA expression
• Identification of novel small RNAs
• Identification of target molecules of novel miRNAs

Chromatin Immunoprecipitation (ChIP) is an experimental technique used to investigate the interaction between proteins and DNA in eukaryotic cells. It aims to determine whether specific proteins are associated with specific genomic regions, such as transcription factors on promoters or other DNA binding sites.

High-throughput sequencing on Illumina HiSeq 2000 is the state-of-the-art method to analyse ChIP experiments. We prepare a short insert cDNA library for each sample and subsequently sequence libraries in a single channel using Illumina HiSeq 2000 technology. DNA-protein interactions of several samples can be analysed in parallel, as we offer pooling of up to 12 samples.

Typical projects are:

• Identification of DNA binding sites of transcription factors and other chromatin associated proteins
• Mapping of DNA binding sites of histone modifiers

Depending on your final objective, we will assist you in designing the appropriate small RNA or ChiP sequencing experiment for your research.

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