Coverage sequencing or complete sequencing of unknown bacterial or fungal genomes or sequencing of metagenomes. After generating the draft data with the latest Genome Sequencer™ FLX technology, we offer scaffolding of contigs and gap closing based on Sanger technology. Comparative sequencing in combination with our Bioinformatic Services allows you to compare your strain of interest with known genomes.

• Sequencing of prokaryotic and eukaryotic genomes of any size
• Identification of organisms that have not been sequenced before
• Deep insight into metagenomes
• Comparison of wild type and mutant strains
• Preparation for metabolic engineering and many other applications
• Stepwise design of the project in close cooperation with you:
  
  Phase I
  Ultra high throughput sequencing with the GS FLX up to 15-20 fold coverage and automatic assembly
  
  Phase II
  Scaffolding of contigs by paired end sequencing with the GS FLX or alternatively by sequencing the ends of a large insert library by ABI technology and scaffolding by co-assembly of the data
  
  Phase III
  Closing of gaps either by means of primer design and sequencing by primer walking on selected clones, or sequencing PCR products of the genomic DNA
  
  Phase IV
  Bioinformatic analysis – e.g. annotation or strain comparison.

• Ultra high throughput of up to 400,000 sequence reads in 7.5 hours
• Sequence reads of 220 to 250 base pairs, resulting in up to 100 Mb of sequence per run
• No cloning needed for Genome Sequencer FLX sequencing
  - no cloning bias especially in AT rich regions
  - full stops in GC rich regions are avoided
  - very even distribution of sequence reads over the complete genome
  - no issues with pathogens or biohazards
  - bio security and bio safety is secured
• Affordable re-sequencing option of production strains
• Re-sequencing instead of other screening methods.