New Customer? Forgot Password?
No SSL
English
 

 
 

 

Fast and Cost-Efficient Sequencing of Small|Genomes and Large Insert Constructs

  • Cost efficient sequencing of small genomes and large insert constructs on Roche GS FLX using an advanced multiplex approach
  • Fast turnaround times supported by our flexible set-up of Roche GS FLX and GS Junior instruments
  • First rate assembly quality supported by the long read lengths of the Roche GS FLX technology

You can rely on our experience in sequencing all kinds of small genomes such as viruses, phages, or organelle DNA (e.g. mitochondria, chloroplasts).

The successful completion of more than 2000 BAC projects has built our expertise in sequencing all kinds of large insert constructs like

  • BACs
  • PACs
  • Cosmids
  • Fosmids

General workflow of your project:

  1. Growing and preparing of clones for sequencing of large insert constructs
  2. Generation of the shotgun library
  3. High throughput sequencing with at least 15-20-fold coverage
  4. Assembly of the sequence reads

Optional parts of our service include closing of gaps based on Sanger technology and bioinformatic analysis such as mapping to a reference, annotation, or ORF calling.

Small genome & large insert construct sequencing projects|– Smart solutions for your project

Depending on your final objective, we will assist you in designing the appropriate sequencing experiment for your research.

Are you interested in an individual quote? You need more information? Just contact us! >


 

Latest Blog Posts

Expression Profiling with 3‘-Libraries

>> Read more

Expression Profiling and Sequencing Depth

>> Read more

Spring Special – Amplicon Sequencing

>> Read more

Related Links

>> Publications
Get list of publications

>> Literature
Download brochure, flyers & application notes

>> FAQs
Find answers on frequently asked questions

Expanded Capacities

To address special needs for small projects, we have expanded our capacity with Roche GS Junior sequencer.

>> Read more