- Cost efficient sequencing of small genomes and large insert constructs on Roche GS FLX using an advanced multiplex approach
- Fast turnaround times supported by our flexible set-up of Roche GS FLX and GS Junior instruments
- First rate assembly quality supported by the long read lengths of the Roche GS FLX technology
You can rely on our experience in sequencing all kinds of small genomes such as viruses, phages, or organelle DNA (e.g. mitochondria, chloroplasts).
The successful completion of more than 2000 BAC projects has built our expertise in sequencing all kinds of large insert constructs like
General workflow of your project:
- Growing and preparing of clones for sequencing of large insert constructs
- Generation of the shotgun library
- High throughput sequencing with at least 15-20-fold coverage
- Assembly of the sequence reads
Optional parts of our service include closing of gaps based on Sanger technology and bioinformatic analysis such as mapping to a reference, annotation, or ORF calling.
Depending on your final objective, we will assist you in designing the appropriate sequencing experiment for your research.
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