Sequencing of cDNA or SAGE clones by Sanger technology with read lengths up to 1,100 base pairs.
Eurofins MWG Operon is offering ultra-deep sequencing of cDNA-, SAGE-, miRNA- and sncRNA-libraries with the Genome Sequencer™ FLX Titanium reagents.
Sequencing of EST or SAGE Clones by Sanger Technology
- Clones can be delivered as stab or glycerol cultures in plates
- Delivery of cDNA libraries as ligation- or transformation mix possible
- High throughput DNA preparation and purification
- High throughput sequencing with Sanger technology
- Read length up to 1100 bases
- Sequencing from the 5’-, the 3’- or both ends
- Professional clustering and assembly
- Additional bioinformatic services on request
- High quality sequence data
- Clones for further experiments available.
Sequencing of cDNA Libraries by GS FLX
- Ultra Deep Sequencing of libraries with GS FLX Titanium reagents
- No cloning required, therefore no cloning bias
- Sequencing from the 5’-, the 3’-end or shotgun sequencing is possible
- No arrays necessary for expression studies
- Detection of transcripts that are not on commercially available arrays.
- Ligation and transformation service included
- Experience of over 100 large EST projects
- cDNA library service available: “From tissue to the transcriptome”
- No array necessary for expression studies.
