Get an excellent picture of whole transcripts by sequencing of our random primed cDNA libraries. To sequence the transcriptome as comprehensively as possible, the libraries are normalised to equalize transcripts of varying amounts. With this approach, sequencing can cover whole transcriptomes and even detect rare transcripts.
Our random primed cDNA library is adapted to be sequenced on Roche GS FLX. Using the long read lengths of 350-450 bp, most full size transcripts can be easily assembled.
Your advantages:
- Coverage of the whole transcriptome
- Detection of rare or yet unknown transcripts
- Finding new RNA viruses or anti-sense RNA (for poly(A)-tailed RNA)
Excellent solutions to compare expression levels of transcripts from different samples are available with our proprietary 3’-fragment cDNA library. The library allows directional sequencing - from the end of the coding sequence into the 3’-UTR. Therefore, reads can be easily anchored to a specific position of a reference sequence. Expression levels are then determined by simply counting reads in different samples.
Our 3’-fragment cDNA libraries can be sequenced either on Roche GS FLX (long reads) or on Illumina HiSeq 2000 (short reads). Sequencing on HiSeq 2000 guarantees quantitative ultra deep sequencing and expression profiling.
Depending on your final objective, we will assist you in designing the appropriate transcriptome analysis experiment for your research.
