Unmodified DNA oligonucleotides are synthesised in our high throughput production facility, using highly automated synthesiser technologies in combination with a sophisticated, completely barcode driven LIMS.

Unmodified DNA oligos are available either as Salt Free oligos or as purified oligos. Purifications can be performed either by our proprietary HPSF^® purification method, by HPLC or by HYPUR^® gel purification.

A chemical reaction never processes completely. Impurities which occur during oligonucleotide synthesis have to be removed.
Using phosphoramidite chemistry, the main side reaction is the formation of n-x products. The longer the sequence, the more n-x products occur. After deprotection, the protecting groups will be part of the raw synthesis product.

	Yield [%] CE 99.5%	Yield [%] CE 99.0%	Yield [%] CE 98.5%
20mer	91	83	75
30mer	86	75	65
40mer	82	68	55
60mer	74	55	41
100mer	61	37	22
Theoretical yield of full length product = coupling efficiency (CE) (length of oligonucleotide-1)

Salt Free Oligos

• Deprotected oligonucleotides
• Quality control of each oligo by MALDI-TOF MS

HPSF^® Purification (High Purity Salt Free)

• Proprietary cartridge purification based on reverse phased chromatography
• Cleaned from any toxic chemicals and n-x products
• Quality control of each oligo by MALDI-TOF MS

HPLC Purification (High Performance Liquid Chromatography)

• High purity (> 80%) due to high chromatographic separation
• Standard purification for all modifications (except Amino C6)
• Recommended for RNA oligonucleotides
• Quality control of each oligo by MALDI-TOF MS or CGE (> 60mers)

HYPUR^® Purification (Purification by Gel Electrophoresis)

• Highest available quality for longmers: > 90% purity
• Recommended for unmodified oligonucleotides > 60mers
• Quality control of each oligo by MALDI-TOF MS (< 60mers) or CGE (> 60mers)

HPSF^® and HYPUR^® are registered trademarks of MWG Biotech AG.