Eurofins MWG Operon: siRNA Modifications

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Modifications

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Eurofinsdna.com > Products & Services > siRNA > siRNA Modifications

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Eurofins MWG Operon offers a wide variety of 5´ and 3´ modifications on either the sense or antisense strands of siRNA duplexes.

Position	5'	3'
FAM	+
HEX	+
TET	+
CY3	+
CY5	+
Amino C6	+
Biotin	+	+
Cholesteryl	+
Phosphate	+
Amino C7		+
Biotin (TEG)		+
Fluorescin		+

Purification	HPLC¹
Purity²	≥ 80%
Guaranteed Yield³	0.05 µmol	5 nmol
	0.20 µmol	15 nmol
	1.0 µM	30 nmol
Turnaround time	5-7 days
¹ Modified siRNA requires HPLC purification ² Purity is based on capillary gel electrophoresis (CGE) analysis of the single strand before the duplex is annealed ³ Yield and purity may be lower for sequences with higher then 70% GC content, containing three or more purine stretches, or having strong secondary structure