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FAQs Oligonucleotides
What kind of oligonucleotide services does MWG offer?
Which synthesis scales does MWG offer?
What does HPSF® mean?
When do I need HPLC Purification?
What is MWG´s HYPUR® Purification?
Which quality control measures are used at MWG?
Which modifications does MWG offer?
Can I have different backbone modifications in one oligonucleotide?
Can I have different modifications in one oligonucleotide?
Which dye combinations are possible for Dual Labelled Probes or Molecular Beacons?
How can I order DNA & RNA oligonucleotides?
How can I order degenerated/wobble bases?
How can I specify modifications?
How can I order oligonucleotides with phosphorothioate bonds?
Can I upload a text or excel file into the ecommerce system?
Can I order modified RNA?
How can I cancel or amend an order?
How can I track my order?
How does MWG produce oligos and which chemistry is used?
Does the sequence of an oligo have an impact on synthesis success?
What is the minimum and maximum oligo length being synthesised?
Why is it necessary to purify oligonucleotides?
Are all MWG oligonucleotides purified by HPSF®?
Which purification method offered by MWG is recommended for my individual application?
What can I do when the oligo doesn’t work in my PCR?
What is the oligo synthesis yield?
What does OD stand for?
Why is the yield sometimes not reproducible?
How long are unmodified oligonucleotides stable and how should I store them?
What is the shelf life of HPSF® oligonucleotides?
How can I optimise the shelf life of HPSF® oligos?
How should fluorescent oligonucleotides be stored?
How should I resuspend my oligonucleotides in tubes and in plates?
How do I calculate the extinction coefficient of an oligonucleotide?
How do I calculate the molar amount N of an oligo from the OD value?
How do I calculate the mass amount m from the molar amount N of an oligo?
How do I calculate the molecular weight M of an oligonucleotide?
How do I calculate the melting temperature TM?
How do I calculate the annealing temperature TA?
How do I anneal complementary oligos?
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