For single sequences, our online Design Tools enable you to define specific primers by yourself.

Our PCR Primer Design Tool suggests a range of optimal primers or pairs of primers for PCR, RT-PCR or sequencing reactions, using a set of physical parameters. The primers are scored in relevance to the predicted best performance criteria. The most favourable primer can be found at the first position.

The PCR Primer Design Tool can also be combined with a database search. The primers are checked against the RefSeq mRNA database (NCBI) to make sure that the primers have no critical mismatches.