Reliable Design for efficient Silencing Results
The online siMAX™ Design Software of Eurofins MWG Operon combines advanced design features with full transparency. The software is a flexible open system with the ability to customise the design processes of our customers. The design program is linked to our online ordering system, facilitating a seamless transition from the design to the purchase of siRNA

Basic Criteria
The siMAX™ Design is based on published data including, among others, Reynolds et al. Nature Biotechnology. The siRNA candidates are ranked based on 12 decisive criteria, including GC content or the specific occurrence of nucleotide bases in critical positions of the siRNA motif. These default criteria can be adjusted in the screen set up if needed.

Secondary Structure of the mRNA
Eurofins MWG Operon has been the first siRNA supplier who offered design features including the preview of the secondary structure formation of the siRNA/mRNA site. Our free online Design Tool has been optimised to show the top folding probability of each target site.


Examples of siRNA target sites (red) on the corresponding mRNA secondary structure predicted using RNAfold. (a) Target site enclosed by two neighbouring hairpin structures. (b) Target site on a region with three internal loops. (c) Target site on a stack region.