If you use oligonucleotides as primers for PCR, RT-PCR , probes for qPCR applications or sequencing, the result of your experiment strongly depends on the properties of your oligos.
Physical properties are:

• the number of bases (length of oligo)
• the series of bases (sequence of oligo)
• the proportion of guanine and cytosine bases of a sequence (GC content)

Other parameters which should be considered are free hydrogen bonds which lead oligos to

• form structures and / or
• build self-dimers or dimers with other oligos

Our online Oligo Design Tools help you to define optimal oligos for your application in advance, avoiding the failure of your experiment due to any unfavourable properties of your oligonucleotides.
Whenever you plan experiments where you need a bulk of oligos or you have other specific design needs, the experts at Eurofins MWG Operon will help you managing your project via our Oligo Design Project Services